Distribution of G143A Mutations Conferring Fungicide Resistance in Virginia Populations of <i>Parastagonospora nodorum</i> Infecting Wheat
نویسندگان
چکیده
Stagonospora nodorum blotch (SNB) caused by Parastagonospora is an important leaf spot disease in the mid-Atlantic United States. Disease management approaches include use of resistant varieties, cultural control, and foliar fungicides. Frequent fungicides can select for fungicide resistance within pathogen populations. Recently, first report quinone outside inhibitor (QoI) States was made based on a relatively small collection P. isolates from Virginia. The objective this study to conduct statewide, 2-year survey populations Virginia wheat quantify frequencies target-site mutation that confers QoI resistance. A total 318 were obtained collected at seven locations distributed throughout wheat-growing regions 2018 2019. previously designed pyrosequencing assay detects G143A substitution cytochrome b gene used screen presence or absence target site mutation. detected all sampled fields. Among years, ranged 5 32% (mean = 19%). Thus, QoI-resistance-conferring widespread Virginia, it occurred high frequency. Results suggest containing active ingredients may not be effective controlling SNB surrounding region, application standalone control recommended.
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ژورنال
عنوان ژورنال: Plant Health Progress
سال: 2022
ISSN: ['1535-1025']
DOI: https://doi.org/10.1094/php-05-21-0082-rs